Interleukin 7, Recombinant, Human (Interleukin-7, IL7, IL-7)

Source:\nRecombinant corresponding to 172aa of human Interleukin-7 expressed in E.coli is a single, non-glycosylated polypeptide chain.\n\nMolecular Weight:\n~17.4kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of mouse IXN/2B cellsis less then 0.5ng/ml, corresponding to a Specific Activity of 2,000,000 IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8429-10F

Size

10 Vials

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Disulfide bond assignment in human interleukin-7 by matrix-assisted laser desorption/ionization mass spectroscopy and site-directed cysteine to serine mutational analysis. J Biol Chem 1997 Dec 26;272(52):32995-3000. 2. Homology modeling study of the human interleukin-7 receptor complex. Protein Eng 1996 Dec;9(12):1135-42. 3. Quantitative and cell-cycle differences in progenitor cells mobilized by recombinant human interleukin-7 and recombinant human granulocyte colony-stimulating factor. Blood 1996 Dec 1;88(11):4139-48. 4. Prediction of the three-dimensional structure of human interleukin-7 by homology modeling. Protein Eng 1996 Jun;9(6):493-8. 5. Mobilization of long-term reconstituting hematopoietic stem cells in mice by recombinant human interleukin 7. J Exp Med 1995 Jan 1;181(1):369-74. 6. Consequences of stable transduction and antigen-inducible expression of the human interleukin-7 gene on tetanus-toxoid-specific T cells. Hum Gene Ther 1994 Dec;5(12):1457-66.