Interleukin 7, Recombinant, Mouse (IL-7)

Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis since it sustains thymocyte proliferation and survival. It regulates peripheral naive T cell survival by modulating the expression of the anti-apoptotic molecule Bcl-2, and sustains peripheral T cell expansion in response to antigenic stimulation. Infection by the human immunodeficiency virus (HIV) leads to severe T lymphopenia and general immune dysfunction. Increased IL-7 plasma levels are generally observed in HIV-infected patients.\n\nDescription: \nRecombinant Murine IL-7 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 14897 Dalton. Recombinant Mouse IL-7 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Cys-His-Ile.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nMurine IL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine 2E8 cells is < 0.2ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Murine IL-7.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of Murine IL-7 as a Reference Standard.\n\nGene: \nName: Il7; Synonyms: Il-7\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Murine IL-7 in sterile 18M -cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Recombinant Murine IL-7 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Murine IL-7 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8429B

Size

2ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Effects of human interleukin 7 on HIV-1 replication in monocyte-derived human macrophages.\n\nAIDS 2006 Apr 4;20(6):937-9\n\n2. Interleukin-7 levels before highly active antiretroviral therapy may predict CD4+ T-cell recovery and virological failure in HIV-infected children.\n\nJ Antimicrob Chemother 2006 Apr;57(4):798-800\n\n3. Increased plasma interleukin-7 level correlates with decreased CD127 and Increased CD132 extracellular expression on T cell subsets in patients with HIV-1 infection.\n\nJ Infect Dis 2006 Feb 15;193(4):505-14\n\n4. The biological role and potential therapeutic application of interleukin 7.\n\nArch Immunol Ther Exp (Warsz) 2005 Nov-Dec;53(6):518-25\n\n5. Ex vivo expansion of dendritic-cell-activated antigen-specific CD4+ T cells with anti-CD3/CD28, interleukin-7, and interleukin-15: potential for adoptive T cell immunotherapy.\n\nClin Immunol 2006 Apr;119(1):21-31\n\n6. Interleukin-7 is a survival factor for CD4+ CD25+ T-cells and is expressed by diabetes-suppressive dendritic cells.\n\nDiabetes 2006 Jan;55(1):158-70