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Interleukin 7, Recombinant, Rat (IL-7)

Cat no: I8429-28

Interleukin 7, Recombinant, Rat (IL-7)

IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by\nthymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant rat IL-7 is a 15.0kD protein containing 130aa residues.\n\nSource:\nRecombinant corresponding to aa130 expressed in E.coli.\n\nSpecific Activity:\nThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine 2E8\ncells is < 2ng/ml, corresponding to a specific activity of >5x106 units/mg.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8429-28

Size

10ug

Applications

ELISA

Form

Supplied as a lyophilized powder with no additives. Reconstitute in sterile ddH2O to 0.1-1ug/ul.

Purity

~98% (SDS-PAGE and HPLC.) Endotoxin: <0.1ng/ug.

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