Interleukin 8, aa1-72, Recombinant, Porcine (IL-8)

Recombinant Porcine IL-8 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 72 amino acids and having a molecular mass of 8291 Dalton. Porcine IL-8 is purified by proprietary chromatographic techniques.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity: \nPorcine IL-8 is fully biologically active when compared to standard. The biological activity of IL-8 was determined by the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils and was found to be >1.0 ng/mL.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Porcine IL-8.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.638 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).\n2. Analysis by RP-HPLC, using a standard solution of IL-8 as a Reference Standard.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Porcine IL-8 in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Recombinant Porcine IL-8 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Porcine IL-8 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8430D

Size

5ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Melanoma cell extravasation under flow conditions is modulated by leukocytes and endogenously produced interleukin 8.\n\nMol Cell Biomech 2005 Sep;2(3):145-59\n\n2. Interleukin-8 levels in seminal plasma in chronic prostatitis/chronic pelvic pain syndrome and nonspecific urethritis.\n\nBJU Int 2006 May;97(5):1043-6\n\n3. Nonclassical pathway of Pseudomonas aeruginosa DNA-induced interleukin-8 secretion in cystic fibrosis airway epithelial cells.\n\nInfect Immun 2006 May;74(5):2975-84\n\n4. Interleukin-8: more on the mechanisms of progenitor cells mobilization in acute coronary syndromes.\n\nEur Heart J 2006 May;27(9):1013-5\n\n5. Urine interleukin-8 as a marker of vesicoureteral reflux in infants.\n\nPediatrics 2006 May;117(5):e863-7\n\n6. Time-dependent alterations in gene expression of interleukin-8 in the bronchial epithelium of horses with recurrent airway obstruction.\n\nAm J Vet Res 2006 Apr;67(4):669-77