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Interleukin 8, Endothelial, Recombinant, Human (NAP-1)

Cat no: I8430-27H

Interleukin 8, Endothelial, Recombinant, Human (NAP-1)

Source: \nRecombinant corresponding to 77aa from human IL-8 expressed in E. coli.\n\nMolecular Weight: \n~8.9kD\n\nActivity:\nThe biological activity of this protein was determined by quantitating dose dependent chemotaxis with human peripheral blood neutrophils in a modified Boyden chamber. Chemotaxis was observed at a concentration range of 25-150 ng/ml. The optimal concentration should be determined for each specific application. This 77 amino acid residue form of IL-8 is the predominant form secreted by endothelial cells.\n\nEndotoxin: ~0.1ng/ug\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8430-27H

Size

25ug

Form

Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Purity

~98% (SDS-PAGE, HPLC)

References

1. Wang, B.N., E. Kraig, and D. Kolodrubetz (2000) Use of defined mutants to assess the role of the Campylobacter rectus S-layer in bacterium-epithelial cell interactions. Infection and Immunity 68(3):1465-1473. 2. Horvath-Arcidiacono, J.A. and E.T. Bloom (2001) Characterization of human killer cell reactivity against porcine target cells: differential modulation by cytokines. Xenotransplantation 8(1):62-74.

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