Involucrin

Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix.\n\nApplications:\nSuitable for use in ELISA (For coating, use antibody without BSA), Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunoprecipitation: 2ug/mg protein lysate (Denatured verified; Use Protein G).\nWestern Blot: 1-2ug/ml for 2 hours at RT.\nImmunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. For staining formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, for 10 minutes at 37 degrees C or Protease XXV at 1mg/ml PBS, for 5 minutes at 37 degrees C. \nNote: Enzyme digestion is better than citrate buffer based epitope unmasking.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nLS174T cells and skin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I8447-25

Size

500ul

Applications

ELISA, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat, Can, Prc

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See I8447-25X.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Hudson, D.L., et al., Hybridoma 1992, 11:367

Additional Info

Recognizes human involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes: 170kD in MCF-7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in canine, and a doublet of 105kD in porcine. Species Crossreactivity: gorilla, owl monkey, porcine, canine and rat