IP-10, Recombinant, Human (Interferon gamma Inducible Protein, CXCL10)

Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto's thyroiditis, Graves'disease and Type 1 diabetes mellitus.The chemokine IP-10 (interferon-inducible protein of 10 kD, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells\n\nDescription: \nRecombinant Human IP-10 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8516 Dalton. Recombinant Human IP-10 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe first five N-terminal amino acids is Val-Pro-Leu-Ser-Arg.\n\nBiological Activity: \nRecombinant Human IP-10 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human T lymphocytes at 10-100.0 ng/ml.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of IP-10.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\n\n\n\n\n

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SPECIFICATIONS

Catalog Number

I8449-08N

Size

5ug

Form

Supplied as a lyophilized powder without additives. Reconstitute with sterile, ddH2O, 0.1% BSA or HSA to (same/more than)100ug/ml

Purity

(same/more than) 98% as determined by RP-HPLC, and/or reducing and non-reducing SDS-PAGE Silver Stained gelEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug).

References

1. Early post-transplant urinary IP-10 expression after kidney transplantation is predictive of short- and long-term graft function. Kidney Int 2006 May;69(9):1683-90 2. IP-10 blocks vascular endothelial growth factor-induced endothelial cell motility and tube formation via inhibition of calpain. Circ Res 2006 Mar 17;98(5):617-25 3. Compartment-specific expression and function of the chemokine IP-10/CXCL10 in a model of renal endothelial microvascular injury. J Am Soc Nephrol 2006 Feb;17(2):454-64 4. IP-10/MCP-1 ratio in CSF is an useful diagnostic marker of neuropsychiatric lupus patients. Rheumatology (Oxford) 2006 Feb;45(2):232-4 5. T lymphocyte chemotactic chemokines in acute myelogenous leukemia (AML): local release by native human AML blasts and systemic levels of CXCL10 (IP-10), CCL5 (RANTES) and CCL17 (TARC). Cancer Immunol Immunother 2006 Jul;55(7):830-40 6. TLR3-mediated signal induces proinflammatory cytokine and chemokine gene expression in astrocytes: differential signaling mechanisms of TLR3-induced IP-10 and IL-8 gene expression. Glia 2006 Feb;53(3):248-56