IP-10, Recombinant, Mouse (Gamma Interferon Inducible Protein-10)

Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto's thyroiditis, Graves'disease and Type 1 diabetes mellitus.The chemokine IP-10 (interferon-inducible protein of 10 kD, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells.\n\nDescription: \nRecombinant Mouse IP-10 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8701Dalton. Recombinant Murine IP-10 is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Ile-Pro-Leu-Ala-Arg.\n\nDimers and Aggregates: \nLess than 1% as determined by silver stained SDS-PAGE gel analysis.\n\nBiological Activity: \nMurine IP-10 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract IL-2 activated T cells at 0.1-10 ng/ml.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Mouse IP-10.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm using the absorbency value of 0.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).\n2. Analysis by RP-HPLC, using a standard solution of IP-10 as a Reference Standard.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Mouse IP-10 in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized IP-10 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IP-10 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

I8449-12A

Size

5ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 97% as determined by: \n(a) Analysis by RP-HPLC. \n(b) Anion-exchange FPLC. \n(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

References

1. Early post-transplant urinary IP-10 expression after kidney transplantation is predictive of short- and long-term graft function.\n\nKidney Int 2006 May;69(9):1683-90\n\n2. IP-10 blocks vascular endothelial growth factor-induced endothelial cell motility and tube formation via inhibition of calpain.\n\nCirc Res 2006 Mar 17;98(5):617-25\n\n3. Compartment-specific expression and function of the chemokine IP-10/CXCL10 in a model of renal endothelial microvascular injury.\n\nJ Am Soc Nephrol 2006 Feb;17(2):454-64\n\n4. IP-10/MCP-1 ratio in CSF is an useful diagnostic marker of neuropsychiatric lupus patients.\n\nRheumatology (Oxford) 2006 Feb;45(2):232-4\n\n5. T lymphocyte chemotactic chemokines in acute myelogenous leukemia (AML): local release by native human AML blasts and systemic levels of CXCL10 (IP-10), CCL5 (RANTES) and CCL17 (TARC).\n\nCancer Immunol Immunother 2006 Jul;55(7):830-40\n\n6. TLR3-mediated signal induces proinflammatory cytokine and chemokine gene expression in astrocytes: differential signaling mechanisms of TLR3-induced IP-10 and IL-8 gene expression.\n\nGlia 2006 Feb;53(3):248-56