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IPP-1, aa1-171, Recombinant, Human, His-tag (Inhibitor of Protein Phosphatase)

Cat no: I8465-02B

IPP-1, aa1-171, Recombinant, Human, His-tag (Inhibitor of Protein Phosphatase)

IPP-1, 1-171aa, Human, His-tagged, Recombinant, E. coli\n\nFull name: Protein phosphatase inhibitor-1 \n\nNCBI Accession: NP_006732 \n\nSynonyms: IPP-1, I-1, PPI-1\n\nProtein phosphatase inhibitor-1 (IPP-1) plays an important role in the regulation of glycogen metabolism through inhibition of type-1 protein serine/threonine phosphatase(PP1) activity, and it has been implicated in the regulation of cell growth. IPP-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. In the presence of calcium, PPI-1 is inactivated by calcineurin (or PP2B). \n\nRecombinant human IPP-1, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.\n\nMolecular Weight: 20kD (179 aa)\n\nSequence: MEQDNSPRKI QFTVPLLEPH LDPEAAEQIR RRRPTPATLV LTSDQSSPEI \nDEDRIPNPHL KSTLAMSPRQ RKKMTRITPT MKELQMMVEH HLGQQQQGEE \nPEGAAESTGT QESRPPGIPD TEVESRLGTS GTAKKTAECI PKTHERGSKE \nPSTKEPSTHI PPLDSKGANS VLEHHHHHH\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8465-02B

Size

50ug

Form

Supplied as a liquid in 50mM Tris, pH 8.0, 0.1mM PMSF, 1mM EDTA, 1mM DTT, 10% glycerol.

Purity

(same/more than) 90% by SDS PAGE

References

Weiser DC., et al. (2004), J. Biol. Chem. Nov 19;279(47):48904-14\nRodriguez P., et al. (2006), J. Biol. Chem. Dec 15;281(50):38599-608

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