ITGB1BP1 (ICAP1, Integrin beta-1-binding Protein 1, Integrin Cytoplasmic Domain-associated Protein 1, ICAP-1)

ICAP-1 is a 22-27kD protein that specifically binds to the cytoplasmic tail of B1 integrin. It is widely expressed, and serves to negatively regulate cell migration/proliferation. In the cytosol, it has at least two functions. First, it associates withz B1 integrin, promoting an inactive conformation. This has the effect of both slowing cell migration and allowing for the monitoring of ECM density. Second, it induces Delta-Notch signaling, an event that specifies the growth of only tip endothelium during angiogenesis. In the nucleus, ICAP-1 is proposed to participate in c-myc activation. Human ICAP-1 is 200aa in length. It contains an NLS aa5-9 and one pleckstrin homology-like/PID domain aa58-200. Phosphorylation occurs at aa38-41. There is one nonintegrin-binding alternative splice form that shows a deletion of aa128-177. Over aa1-127, human ICAP-1 shares 93% aa identity with mouse ICAP-1.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nImmunohistochemistry: 5-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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