J Chain

Human J chain is a polypeptide folded within the polymeric structure of the immunoglobulin. J chain isolated from human polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain has been established as distinct from all other component chains of polymeric IgA and IgM. It has a unique primary structure. Antisera to light chains or heavy chains of human Immunoglobulins do not react with J chain. J chain is released from dimeric human myeloma IgA by sulphitolysis and purified by ion exchange chromatography in urea and gel filtration in guanidine. Its purity is tested in alkaline urea polyacrylamide gel\nelectrophoresis, in immunodiffusion and immunoelectrophoresis, showing it to be free of IgA, heavy or light chain contaminants. \n\nApplications: \nSuitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

J1255-01

Size

1ml

Applications

ELISA

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

Additional Info

Recognizes human J chain. In immunoelectrophoresis and radial immunodiffusion shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA and IgM. No reaction is obtained with the intact polyclonal or monoclonal IgA, IgM and IgG. There is also no detectable reaction with normal human serum, normal human milk, purified Ig heavy or light chains or secretory component. However a single precipitin line can be obtained with human serum after two hours incubation with 9M urea and 0.2M mercaptoethanol at pH 8.6.