Jagged1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille syndrome protein)

The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors. JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis. JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia. Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro. JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro. Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome.\n\nApplications:\nSuitable for use in ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

J0400-01

Size

50ug

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Ropke A, Kujat A, Graber M, Giannakudis J, Hansmann I: Identification of 36 novel Jagged1 (JAG1) mutations in patients with Alagille syndrome. Hum. Mutat. 100 (2003).\n1. John GR, Shankar SL, Shafit-Zagardo B, Massimi A, Lee SC, Raine CS, Brosnan CF: Multiple sclerosis: re-expression of a developmental pathway that restricts oligodendrocyte maturation. Nat. Med. 1115-1121 (2002).\n1. Kohsaka T, Yuan ZR, Guo SX, Tagawa M, Nakamura A, Nakano M, Kawasasaki H, Inomata Y, Tanaka K, Miyauchi J: The significance of human jagged 1 mutations detected in severe cases of extrahepatic biliary atresia. Hepatology 36(4 Pt 1): 904-912 (2002).\n2. Masuya M, Katayama N, Hoshino N, Nishikawa H, Sakano S, Araki H, Mitani H, Suzuki H, Miyashita H, Kobayashi K, Nishii K, Minami N, Shiku H: The soluble Notch ligand, Jagged-1, inhibits proliferation of CD34+ macrophage progenitors. Int. J. Hematol. 75(3): 269-276 (2002).\n3. Piccoli DA, Spinner NB: Alagille syndrome and the Jagged1 gene. Semin. Liver Dis. 21(4): 525-534 (2001); review.\n4. Gray GE, Mann RS, Mitsiadis E, Henrique D, Carcangiu ML, Banks A, Leiman J, Ward D, Ish-Horowitz D, Artavanis-Tsakonas S: Human ligands of the Notch receptor. Am. J. Pathol. 154(3): 785-794 (1999).\n5. Lindsell CE, Shawber CJ, Boulter J, Weinmaster G: Jagged: a mammalian ligand that activates Notch1. Cell 80(6): 909-917 (1995). PMID: 7697721 [PubMed - indexed for MEDLINE]

Additional Info

Recognizes human Jagged1 (JAG1).