JAK1, phosphorylated (Tyr1022, Tyr1023) (Janus Kinase 1)

Janus Kinase 1 (JAK1) is a 130kD tyrosine kinase involved in cytoplasmic signal transduction. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1022 and 1023 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls: \n3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

J0900-20B

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, (without Mg2+ and Ca2+), pH 7.3, 50% glycerol, 1mg/mL BSA (IgG, protease free), 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

References

1. Fukada, T. and N.K. Tonks (2003) Identification of YB-1 as a regulator of PTP1B expression:\nimplications for regulation of insulin and cytokine signaling. EMBO J. 22(3):479-493 (cites the use\nof this antibody). 2. Booz, G.W., et al. (2002) Cytokine G-protein signaling crosstalk in cardiomyocytes: attenuation of Jak-STAT activation by endothelin-1. Mol. Cell. Biochem. 240(1-2):39-46 (cites the use of cat# 44-380, 44-400 and 44-422). 3. Ju, H., et al. (2000) Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem. J. 351(Pt 1):257-264.\n4. Liu, K.D., et al. (1997) Janus kinases in interleukin-2-mediated signaling: JAK1 and JAK3 are differentially regulated by tyrosine phosphorylation. Curr. Biol. 7(11):817-826. 5. Ihle, J.N. (1996) STATs: signal transducers and activators of transcription. Cell 84(3):331-334.

Additional Info

Recognizes human and mouse JAK1 when phosphorylated at Tyr1022, Tyr1023. Species sequence homology: porcine. Does not react with rat JAK1.\n