JAK2 (Janus Kinase 2) (Protein A Agarose)

Polyclonal Rabbit anti-Mouse JAK2 (Janus Kinase 2) antibody (Catalog No: J0901-04) covalently coupled to Protein A Agarose by dimethylpimelimidate.\n\nThe Janus (JAK) family of cytoplasmic tyrosine kinases has been implicated in signal transduction induced by cytokines and growth factors, physically associated with ligand-bound receptors. This association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130kD and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members. Family members such as JAK1, JAK2, and TYK2 are ubiquitous, whereas JAK3 is predominantly expressed in T lymphocytes. Studies with mutant cells that do not express JAK1, JAK2, or TYK2 show that their activation is essential for cellular signaling. JAK family kinases may either phosphorylate each other or be phosphorylated by a yet undescribed tyrosine kinase. Different cytokines can activate via phosphorylation distinct JAK family members. In some cells, the membrane-associated gp130 protein has been implicated in the induction of JAK family phosphorylation, although gp130 itself has no known activity. \n\nApplications:\nSuitable for use in Immunoprecipitation and Affinity Purification. Other applications not tested.\n\nRecommended Dilutions:\nImmunoprecipitation: 20ul gel slurry. Immunoprecipitates JAK2 from RIPA lysates of IGF-1 stimulated rat L6 cells as shown by Immunoblot analysis of the immunoprecipitate with JAK2 (Janus Kinase 2) Pab Rb xMo (Catalog No: J0901-04).\nAffinity Purification: The antibody-agarose may be useful for affinity purification of proteins that bind to the specific antigen.\nOptimal dilutions to be determined by researcher.\n\nElution Profile:\nElution Buffer Released Relative % Antigen\nSDS-PAGE sample buffer 100\n0.1M Glycine, pH 2.5 Negligible\n0.1M TEA, pH 11.5 Negligible\n3.5M MgCl2, pH 7.2 Negligible\n\nStorage and Stability:\nStore at 4 degrees C. Stable for at least 12-12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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