JAK2, phosphorylated (Tyr1007, Tyr1008) (Janus Kinase 2)

Janus Kinase 2 (JAK2) is a 130kD tyrosine kinase involved in cytoplasmic signal transduction.\nLigand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to\nan association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation.\n\nApplications: \nSuitable for use in Western Blot.. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \n3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

J0901-10B

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Frank, G.D., et al. (2003) Distinct mechanisms of receptor and nonreceptor tyrosine kinase activation by reactive oxygen species in vascular smooth muscle cells: role of metalloprotease and protein kinase C-delta. Mol. Cell. Biol. 23(5):1581-1589. 2. Kaszubska, W., et al. (2002) Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line. Mol. Cell. Endocrinol. 195(1-2):109-118.\n3. Kirito, K., et al. (2002) Thrombopoietin regulates Bcl-xL gene expression through Stat5 and phosphatidylinositol 3-kinase activation pathways. J. Biol. Chem. 277(10):8329-8337 (cites the use of this antibody). 3. Shaw, S., et al. (2002) Janus kinase 2, an early target of alpha 7 nicotinic acetylcholine receptor-mediated neuroprotection against Abeta-(1-42) amyloid. J. Biol. Chem. 277(47):44920-44924 (cites the use of this antibody). 4. Sutherland, C.L., et al. (2002) UL16-binding proteins, novel MHC class I-related proteins, bind to NKG2D and activate multiple signaling pathways in primary NK cells. J. Immunol. 168(2):671-679 (cites the use of this antibody). 5. Zeng, Z.Z., et al. (2002) 5(S)-hydroxyeicosatetraenoic acid stimulates DNA synthesis in human microvascular endothelial cells via activation of Jak/STAT and phosphatidylinositol 3-kinase/Akt signaling, leading to induction of expression of basic fibroblast growth factor 2. J. Biol. Chem. 277(43):41213-41219 (cites the use of this antibody). 6. Eguchi, S., et al. (2001) Activation of MAPKs by angiotensin II in vascular smooth muscle cells. Metalloprotease-dependent EGF receptor activation is required for activation of ERK and p38 MAPK but not for JNK. J. Biol. Chem. 276(11):7957-7962 (cites the use of this antibody). 7. Madamanchi, N.R., et al. (2001) Thrombin regulates vascular smooth muscle cell growth and heat shock proteins via the JAKSTAT pathway. J. Biol Chem. 276(22):18915-18924 (cites the use of this antibody). 8. Myers, M.P., et al. (2001) TYK2 and JAK2 are substrates of protein-tyrosine phosphatase 1B. J. Biol. Chem. 276(51):47771-47774 (cites the use of this antibody). 9. Madamanchi, N.R., et al. (2000) Reactive oxygen species regulate heat shock protein 70 via the JAK/STAT Pathway. 10. Arteriocher. Thromb. Vasc. Biol. 21(3):321-326 (cites the use of this antibody). 11. Amiri, F., et al. (1999) Hyperglycemia enhances angiotensin II-induced janus-activated kinase/STAT signaling in vascular smooth muscle cells. J. Biol. Chem. 274(45):32382-32386 (cites the use of this antibody). 12. Ihle, J.N. (1996) STATs: signal transducers and activators of transcription. Cell 84(3):331-334.

Additional Info

Recognizes JAK2, phosphorylated (Tyr1007, Tyr1008) (Janus Kinase 2). Species Crossreactivity: human, mouse and rat JAK2. Other species have not been tested.