JAK3, CT (Janus Kinase 3, EC 2.7.10.2, JAKL, Leukocyte Janus Kinase, LJAK)

Jak3 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs translocate to the nucleus to mediate gene transcription (1, 2). Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically IL-2, IL-4, IL-7, IL-9, and IL-15 receptors. Jak3 has been implicated in mast cell-mediated immediate hypersensitivity reactions, autoimmune disorders, and acute lymphoblastic leukemia. Jak3 inhibitor has shown clinical potential as a new class of immunomodulatory agents with anti-leukemic and immunosuppressive properties (3).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:30 \nWestern Blot: 1:2000 \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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