JAK3 (Janus Kinase 3, EC 2.7.10.2, JAKL, Leukocyte Janus Kinase, LJAK)

The Janus (JAK) family of cytoplasmic tyrosine kinases has been implicated in signal transduction induced by cytokines and growth factors, physically associated with ligand-bound receptors. This association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130kD and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members. Family members such as JAK1, JAK2, and TYK2 are ubiquitous, whereas JAK3 is predominantly expressed in T lymphocytes. Studies with mutant cells that do not express JAK1, JAK2, or TYK2 show that their activation is essential for cellular signaling. JAK family kinases may either phosphorylate each other or be phosphorylated by a yet undescribed tyrosine kinase. Different cytokines can activate via phosphorylation distinct JAK family members. In some cells, the membrane-associated gp130 protein has been implicated in the induction of JAK family phosphorylation, although gp130 itself has no known activity. \n \nApplications:\nSuitable for use in Immunoprecipitation and Immunoprecipitation kinase assay. Other applications not tested.\n\nRecommended Dilutions:\nImmunoprecipitation: 5-10ug immunoprecipitated JAK3 from 2mg of RIPA lysate from CTLL cells, previously this antibody has immunoprecipitated JAK3 from a TF-1 RIPA cell lysate. \nImmunoprecipitation Kinase Assay: 5-10ug of this antibody immunoprecipitates JAK3, which can be autophosphorylated in an in vitro kinase reaction. TF1 cell lysate was immunoprecipitated with J0902 (10ug). The immune complex was incubated in kinase buffer containing [y-32 P]ATP and subsequently resolved by electrophoresis, followed by autoradiography.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n

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