JAM-A, Domain 2 (Junctional Adhesion Molecule A, Junctional Adhesion Molecule 1, JAM-1, Platelet F11 Receptor, Platelet Adhesion Molecule 1, PAM-1, CD321, F11R, JAM1, JCAM, UNQ264/PRO301)

Junction adhesion molecule (JAM)-A (also known as JAM, JAM-1 or F11R). Together with JAM-C (JAM-2) and JAM-B (VE- JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A play an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the b2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role of JAM-A in the initiation of atherosclerosis, since JAM-A is upregulated on early atherosclerotic endothelium and adhesion of activated platelets on activated endothelium is mediated by homophilic interactions of JAM- A. The polyclonal antibody reacts with the 17kD extracellular domain 2 of the human JAM-A protein.\n\nApplications:\nSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Paraffin\nFlow Cytometry: 1:50\nWestern Blot: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

J8009-05D

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Fraemohs, L et al; The functional interaction of the beta 2 integrin lymphocyte function-associated antigen-1 with junctional adhesion molecule-A is mediated by the I domain. J Immunol 2004, 173: 6259. 2. Ostermann, G et al; Involvement of JAM-A in mononuclear cell recruitment on inflamed or atherosclerotic endothelium: inhibition by soluble JAM-A. Arterioscler Thromb Vasc Biol 2005, 25: 729. 3. Ostermann, G et al; JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes. Nat Immunol 2002, 3: 151. 4. Zernecke, A et al; Importance of JAM-A for neointimal lesion formation and infiltration in atherosclerosis-prone mice. Arterioscler Thromb Vasc Biol 2006, 26: e10.

Additional Info

Recognizes the second extracellular domain of human JAM-A. Does not react with mouse JAM-A.