JIP 1/2, SH3 (C-Jun-amino-terminal Kinase-interacting Protein 1, JNK-interacting Protein 1, JIP1, JIP-1, C-Jun-amino-terminal Kinase-interacting Protein 2, JNK-interacting Protein 2, JIP2, JIP-2, Islet-brain 1, IB1, IB-1, Islet-brain-2, IB2, IB-2, JNK MAP

The JNK (c-Jun NH2-terminal kinase) signal transduction cascade is one of three identified MAP kinase pathways in mammals. JNK pathway activation occurs in response to environmental stress, is associated with the nuclear accumulation of JNK itself, and is implicated in the immune response, cellular proliferation, oncogenic transformation, morphogenesis, and apoptosis. JIP1 (JNK-interacting protein-1, also known as Islet Brain-1 and mitogen-activated protein kinase 8-interacting protein-1,) is a cytoplasmic protein that possesses an N-terminal JNK-binding domain and a C-terminal SRC homology (SH3) domain. Its SH3 domain is related to the SH3 domains of the tyrosine kinase c-Fyn and the p85 subunit of phosphoinositide-3 kinase (PI3K). Overexpression of JIP1 causes cytoplasmic retention of JNK, thereby preventing its nuclear accumulation and the subsequent activation of the JNK pathway. This observation led to the original hypothesis that JIP1 acts as a cytoplasmic anchor for JNK. JIP1 has been shown to bind selectively to JNK but not to the ERK or p38 MAP kinases; it has also demonstrated selectivity in its binding to specific proteins of the JNK pathway. In addition to JNK, JIP1 binds to the JNK activator MKK7 (MAP kinase kinase 7) and the MKK7 activators MLK3 (mixed-lineage protein kinase 3) and DLK (dual leucine zipper-bearing kinase), all at separate binding sites. JIP1

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SPECIFICATIONS

Catalog Number

J2499-30C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Reacts with human and mouse JIP1 and JIP2. Cross-reactivity with rat JIP1 and JIP2 is expected based on sequence homology. Reactivity has been confirmed with mouse brain homogenates and human embryonic kidney HEK293 cell lysates transfected with full-length JIP1 and the SH3 domain of JIP1. This antibody identifies an ~80kD isoform of the JIP 1 and 2\nproteins in Western blots of mouse brain lysates, and an ~100-110kD isoform in Western blots of cell lines transfected with full-length JIP1 and JIP2.