JMJD1B (KDM3B, Lysine-specific Demethylase 3B, JmjC Domain-containing Histone Demethylation Protein 2B, Jumonji Domain-containing Protein 1B, Nuclear Protein 5qNCA, C5orf7, JHDM2B, KIAA1082)

The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist. This antibody will not cross-react with JMJD1A or JMJD1C.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat Liver Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146503

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Lai F, Godley LA, Fernald AA, et al. cDNA cloning and genomic structure of three genes localized to human chromosome band 5q31 encoding potential nuclear proteins. Genomics 2000; 70:123-30. 2. Hu Z, Gomes I, Horrigan SK, et al. A novel nuclear protein, 5qNCA (LOC51780) is a candidate for the myeloid leukemia tumor suppressor gene on chromosome band q31. Oncogene 2001; 20:6946-54. 3. Baik S-H, Jee B-K, Choi J-S, et al. DNA profiling by array comparative genomic hybridization (CGH) of peripheral blood mononuclear cells (PBMC) and tumor tissue cell in non-small cell lung cancer (NSCLC). Mol. Biol. Rep. 2009; 36:1767-78.

Additional Info

Recognizes human JMJD1B. Species Crossreactivity: mouse and rat.