JMJD1B, NT (JmjC Domain-containing Histone Demethylation Protein 2B, Jumonji Domain-containing Protein 1B, Nuclear Protein 5qNCA, JMJD1B, C5orf7, JHDM2B, KIAA1082, Lysine-specific Demethylase 3B) (Azide free) (HRP)

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:250-1:500 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

J7876-42C-HRP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1.Katoh, M., et al., Int. J. Mol. Med. 12(5):817-821 (2003). 2.Hu, Z., et al., Oncogene 20(47):6946-6954 (2001). 3.Lai, F., et al., Genomics 71(2):235-245 (2001). 4.Lai, F., et al., Genomics 70(1):123-130 (2000). 5.Dias Neto, E., et al., Proc. Natl. Acad. Sci. USA 97(7):3491-3496 (2000).

Additional Info

Recognizes human JMJD1B.