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Jo-1 (Histidyl t-RNA Synthetase)

Cat no: J5000

Jo-1 (Histidyl t-RNA Synthetase)

Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases.\nA heterogeneous group of precipitating antibodies called anti-Jo-1 have been found in dermatopolymyuositis. Anti-Jo is present in approx. 25% of patients with myositis. Recently, Jo-1 has been demonstrated to be an RNA-associated 50kD polypeptide antigen. It has been identified as histidyl-tRNA-synthetase, the RNA being tRNA-his.\nThe frequency of ANA-positives in various rheumatic diseases has been reported for SLE, rheumatoid arthritis (RA), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue diseases, drug-induced SLE, and Sjogren

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SPECIFICATIONS

Catalog Number

J5000

Size

2ml

Applications

ELISA, IP

Reactivities

Hum

Form

Supplied as a lyophilized powder in 0.01M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O or sterile 40-50% glycerol, ddH2O.

P Type

Pab

Purity

Purified by fractionation of delipidated and defibrinated donor human serum.

Isotype

IgG

Additional Info

Recognizes human Jo-1. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. No reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Scl-70, Sm and Sm/RNP antigens in an ELISA system designed to detect all IgG subclasses.

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