JUNB (Transcription Factor Jun-B)

The JunB transcription factor, also known as JunB proto-oncogene, JunB oncogene, transcription factor JunB, and transforming protein JunB, is a basic region leucine zipper DNA-binding protein, and a member of the Jun family of proteins, which also includes c-Jun and JunD. Jun proteins can homodimerize or heterodimerize with other Jun family members, or heterodimerize with Fos family members (cFos, FosB, Fra-1, and Fra-2) to form the activator protein 1 (AP-1) transcription factor. The various dimers differ in their ability to transactivate AP-1-dependent genes. Jun B8,9 and JunD1,10 are almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. JunB and C-Jun antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cyclin A is a direct transcriptional target of JunB driving cell proliferation JunB is an important regulator of erythroid differentiation, and is also important for the development of Th2 effector cells, normal Th2 cytokine expression pattern, and a complete Th2- dependent immune response in mice. JunB expression levels are increased in human colorectal adenocarcinoma specimens as compared to normal mucosa.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:64,000\nWestern Blot: 0.3-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

149866

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Starr JM, Shiels PG, Harris SE, Pattie A, Pearce MS, Relton CL, Deary IJ. Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey. Mechanisms of ageing and development 2008 Dec 129 (12): 745-51.

Additional Info

Recognizes human JUNB at ~35.9kD.