Keratin 13 (Keratin Type I Cytoskeletal 13, Keratin-13, K13, KRT13, Cytokeratin-13, CK13, CK-13)

Cytokeratin 13 is a major component of squamous, non-keratinized epithelium, transitional epithelium, pseudostratified epithelium, and myoepithelium. It is found on tongue, epiglottis, tracheal and esophageal epithelium, and various carcinomas. This antibody will help detect cytokeratin 13 in these tissue types.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:250-1:500\nImmunohistochemistry: 1:100-1:200 for 1 hr. at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nTonsil (for Immunohistochemistry, cytoplasmic staining of mucosa)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K0199-16H

Size

500ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, 1% BSA, 15mM sodium azide. Reconstitute with 0.5ml dH2O.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1.Tanie?re P, Martel-Planche G, Maurici D, et al.. Molecular and clinical differences between adenocarcinomas of the esophagus and of the gastric cardia. American Journal of Pathology. 158(1):33-40 (2001). 2.el-Sissy N A and Rashad N A. CK13 in craniopharyngioma versus related odontogenic neoplasms and human enamel organ. East Mediterranean Health Journal. 5(3):490-502 (1999). 3.Levy R, Czernobilsky B and Geiger B. Subtyping of epithelial cells of normal and metaplastic human uterine cervix, using polypeptide-specific cytokeratin antibodies. Differentiation. 39:185-196 (1988). 4.van Muijen G N P, Ruiter D J, Franke W W, et al.. Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Experimental Cell Research. 162:97-113 (1986). 5.Moll R, Franke W W and Schiller D L. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 31:11-24 (1982).

Additional Info

Recognizes human Cytokeratin 13.