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KIND2 (Fermitin Family Homolog 2, Kindlin-2, Mitogen-inducible Gene 2 Protein, MIG-2, Pleckstrin Homology Domain-containing Family C Member 1, PH Domain-containing Family C Member 1, FERMT2, MIG2, PLEKHC1)

Cat no: K1778-01

KIND2 (Fermitin Family Homolog 2, Kindlin-2, Mitogen-inducible Gene 2 Protein, MIG-2, Pleckstrin Homology Domain-containing Family C Member 1, PH Domain-containing Family C Member 1, FERMT2, MIG2, PLEKHC1)

MIG2 also known as FERMT2 or Kindlin-2, is an ~80kD, 680aa, ubiquitously expressed member of the Fermitin/Kindlin family of cytoplasmic adaptor proteins. MIG2 is essential for B1 and B3 integrin activity due to its interactions with regulatory molecules such as talin and ILK. MIG2 contains two FERM domains aa85-326 and aa524-573 and one pleckstrin homology domain aa373-478. Within the region used as an immunogen, human MIG2 shares 98% aa identity with mouse and rat MIG2. Two isoforms 687aa and 633aa have a 7aa insert after 534aa. The shorter isoform also has a 2aa substitution for the C-terminal 57aa of the 680aa form.\n\nApplications:\nSuitable for use Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K1778-01

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2B

Additional Info

Recognizes human MIG2. Does not react with recombinant human MIG, rhFERMT1 aa514-677, rhMIG2 aa514-680, or rhFERMT3 aa498-667.

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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