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KLF10 (Krueppel-like Factor 10, EGR-alpha, Transforming Growth Factor-beta-inducible Early Growth Response Protein 1, TGFB-inducible Early Growth Response Protein 1, TIEG-1, TIEG, TIEG1)

Cat no: K1891-52D

KLF10 (Krueppel-like Factor 10, EGR-alpha, Transforming Growth Factor-beta-inducible Early Growth Response Protein 1, TGFB-inducible Early Growth Response Protein 1, TIEG-1, TIEG, TIEG1)

LF10 is a 55-65kD member of the Sp1 C2H2-type zinc-finger protein family. It appears to be very widely expressed, and functions as a transcriptional repressor in a noncovalent complex with JARID1B. KLF10 is induced by TGF-B, GDNF and estrogen, and inhibits transcription of genes such as BI-1, OPG and SMAD7. By suppressing SMAD7, it promotes TGF-B signaling. Human KLF10 is 480aa in length and contains three C2H2-type zinc-finger motifs aa369-451. There is one potential alternate splice form that shows a deletion of aa2-11. Over aa31-154, human KLF10 shares 79% aa identity with mouse KLF10/mGIF.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K1891-52D

Size

100ug

Applications

WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human KLF10. Species sequence homology: Recombinant human KLF12 (3%).

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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