KPNA6 (Importin Subunit alpha-7, Karyopherin Subunit alpha-6, IPOA7, FLJ11249, MGC17918)

Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA6 belongs to a subfamily within the KPNA family that also includes IPOA5&6. Down-regulation of KPNA6 by RNAi expression in HeLa cells strongly inhibited cell proliferation, possibly due to blocking the nuclear import of specific factors essential for cell growth and proliferation. KPNA6 also interacts with the Rev protein of HIV-1 and promote its nuclear import.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\n293 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146736

Size

100ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72. 2. Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49. 3. Kohler MC, Fiebeler M, Hartwig S, et al. Evidence for distinct substrate specificity of importin alpha family members in nuclear protein import. Mol. Cell. Biol.1999; 19:7782-91. 4. Quensal C, Friedrich B, Sommer T, et al. In vivo analysis of importin a proteins reveals cellular proliferation inhibition and substrate specificity. Mol. Cell. Biol.2004; 24:10246-55.

Additional Info

Recognizes human KPNA6. Species Crossreactivity: mouse and rat.