Lactate Dehydrogenase A (L-lactate Dehydrogenase A Chain, LDHA, LDH-A, Cell Proliferation-inducing Gene 19 Protein, GSD11, LDH Muscle Subunit, LDHM, LDH-M, LDH1, PIG19, Renal Carcinoma Antigen NY-REN-59)

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle. \n\nApplications:\nSuitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:500\nImmunofluorescence: 1:500-1:8000\nWestern Blot: 1:500-1:3000\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nFO myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L1011-10A

Size

50ul

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG2b,k

References

1. Le A, et al. (2010) Proc Natl Acad Sci U S A, 107(5):2037-42. 2. Xie H, et al. (2009) Mol Cancer Ther, 8(3):626-35. 3. Toyoda K, et al. (2010) J Bacteriol, 191(13):4251-8.\n

Additional Info

Recognizes human LDHA.

Alternative Names

EC=1.1.1.27