Lactogen, Placental (hPL, Choriomammotropin, Chorionic Somatomammotropin A, CSA, Chorionic Somatomammotropin Hormone 1, CSH1, CS1, CSH2, CSMT, hCSA, Somatotropin Precursor)

Human Placental Lactogen (abbreviated PL or hPL), also called chorionic somatomammotropin hormone 1 (abbreviated CSH1), is a member of the prolactin/growth hormone (PRL/GH) family (1). It is found in a cluster of growth hormones on chromosome 17 that appear to have a common ancestry. Identical 191aa mature hPL proteins may be formed from one of two genes (2). PL contains a pair of C-terminal cysteines that may form either intra- or interchain disulfides. Human PL shares 98aa identity with chimpanzee PL and 85aa sequence identity with other human growth hormones, but only 25aa identity with mouse, ovine or bovine PL. PL is mainly expressed by cells in the syncytiotrophoblast layer of the placenta, which produce increasing amounts of PL as pregnancy proceeds. The major portion enters the maternal circulation, where it joins GH2 (placenta-specific GH) in replacing the functions of pituitary GH during pregnancy. A smaller amount of PL circulates in the fetus. Primate PL shows high affinity for the PRL receptor and low affinity for the GH receptor (1). Reduced stimulation of PL by angiotensin 2 correlates with intrauterine growth restriction (3). There is some evidence that mature angiogenic PL may be cleaved to form an anti-angiogenic N-terminal fragment (4). Although PL promotes pancreatic beta cell survival, it does not appear to be altered in gestational diabetes. It helps prepare mammaries for lactation, but probably does not influence lactation itself. PL may be a ligand of stabilin-1, which has been proposed to regulate PL internalization and degradation or re-expression (6).\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nImmunohistochemistry: 8-25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1022-03H

Size

100ug

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

References

1. Handwerger, S. and M. Freemark (2000) J. Ped. Endocrinol. Metab. 13:343. 2. Selby, M.J. et al. (1984) J. Biol. Chem. 259:13131. 3. Szukiewicz, D. et al. (2008) Int. Immunopharmacol. 8:177. 4. Struman, I. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1246. 5. Fujinaka, Y. et al. (2007) J. Biol. Chem. 282:30707. 6. Kzhyshkowska, J. et al. (2008) J. Immunol. 180:3028.

Additional Info

Recognizes human Placental Lactogen. No cross-reactivity with recombinant human Growth Hormone.