LAIR-2 (Leukocyte-associated Ig-like Receptor-2)

Leukocyte Associated Ig-like receptor 2 (LAIR2) is a secreted protein that contains one Ig-like domain, which shares 84% amino acid sequence homology with LAIR1. It lacks the transmembrane ITIM-containing cytoplasmic domains of LAIR1. The function of LAIR2 is not known. An alternate splice form of LAIR2 that shows a 17 amino acid deletion outside the Ig domain exists.\n\nApplications: \nSuitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested\n\nRecommended Dilutions: \nWestern Blot:1-2ug/ml. Using a colorimetric detection system the detection limit is ~5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. \nFlow Cytometry: 25ug/ml. 10ul labels 1-5x10e5 Jurkat cells in a total reaction volume (same/less than)200ul. The cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS.\nDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~5ng/well.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nHybridoma resulting from fusion of mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human LAIR-2 (rhLAIR-2; aa22-152; Accession #Q6ISS4). \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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