Laminin

Laminin is a non-collagenous protein of basement membranes. This glycoprotein has a molecular weight of ~900kD. By rotary shadowing it has a cross-like shape, consisting of one long arm, (400kD) and three short arms (200kD). Antibodies to laminin localize exclusively in basement membranes. Laminin is found throughout the basement membrane, but the reaction with the antibodies to Laminin is more intense in the lamina lucida, the part of the basement membrane adjacent to the cell membrane. Laminin binds to type IV collagen, a component exclusively localized in the basement membrane. Laminin has been shown to play a role in cell adhesion and attachment in vivo and in vitro. \n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen, FFPE): 1:50-1:100 using the Indirect method. For frozen sections fixation with acetone for 5 min at -20 degrees C is recommended. On paraffin embedded tissue sections proteolytic treatment with pepsin is advised. Dilute immediately before use with PBS or Tris.\nOptimal dilutions to be determined by the researcher.\n\nPositive control: \nSkin, kidney\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L1225-01E

Size

250ul

Applications

IHC

Hosts

Rabbit

Form

Supplied as a liquid.

P Type

Pab

Purity

Serum

References

1. Bosman, F.T., et al., 1985, Ultrastructural Pathology 8, 291-304.

Additional Info

Recognizes Laminin. Does not react with human type I, III, IV and V collagen. Reacted with a distinct band of ~200-220kD from a 8M Urea extract from amion basement membrane. Species Crossreactivity: Most mammals