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Laminin

Cat no: L1225-01H

Laminin

Laminin is a large (900kD) complex extracellular glycoprotein and a major component of the basement membrane. Laminin is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin is composed of three different polypeptide chains; alpha, beta and gamma which are bound to each other by disulphide bonds into a cross-shaped molecule. Isoforms have been detected for all three chains. The different combinations of the alpha, beta and gamma chains give rise to at least 15 laminin heterotrimers. \n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 0.25-0.5ug/ml\nImmunohistochemistry (Paraffin and Formalin): 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1225-01H

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Fel, Prc

Form

Supplied as a lyophilized powder in 1.2% sodium acetate, 0.2% sodium azide, 2% BSA. Reconstitute with 1ml sterile PBS (pH 7.4) to 100ug/ml.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Laminin. Species Crossreactivity: feline, porcine.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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