Laminin

Laminin is a glycoprotein (Mr 850-1,000kD, consisting of 3 glycosylated polypeptide chains with molecular weights of 440 and 225 (2x) kD) produced by various human epithelial and\nmesenchymal cells, and forms an extracellular matrix of thin filaments. In normal tissues, laminin is invariably present in all basal laminas surrounding muscle, nerve, fat and decidua cells and separates epithelial and endothelial cells from abutting connective tissues. Laminin has also been identified within the cytoplasm of breast epithelia, stromal cells of the endometrium, and within endothelial, bile duct epithelial and mesenchymal cells of the liver. Laminin has been found to be involved in cellular activities such as adhesion, spreading, differentiation, polarization, proliferation, locomotion, tissue invasion and chemotactic responses.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1:50-1:100\nImmunohistochemistry: 1:50-1:00 Frozen and paraffin tissues (proteolytic treatment required; enzyme: pepsin)\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkin, kidney\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L1225-09

Size

250ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Prc, Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide

P Type

Pab

Purity

Serum

Isotype

IgG

References

Lise Christensen: The distribution of fibronectin, laminin and tetranectin in human breast cancer with special attention to the extracellular matrix. APMIS Supplementum No. 26, Vol. 100 (1992).

Additional Info

Recognizes mouse laminin. Reacts with a distinct band of~200-220kD from a 8M Urea extract from amnion basement membrane. It does not crossreact with human type 1, III, IV and V collagen in Western Blot. Species Crossreactivity: human, bovine, porcine, rat, rabbit and most mammals.