LAMP1 (Lysosome-associated Membrane Glycoprotein 1, LAMP-1, Lysosome-associated Membrane Protein 1, CD107 Antigen-like Family Member A, CD107a)

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP- 2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nEL4 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L9190-10P

Size

50ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Gozuacik, D. et al. Oncogene. 23, 2891 (2004). 2. Kisen, GO. et al. Carcinogenesis 14, 2501 (1993). 3. Kamada, Y. et al. J. Cell. Biol. 150, 1507 (2000). 4. Chen, JW. Et al. J. Cell Biol. 101, 85 (1985). 6. Andrejewski, N. et al. J. Biol. Chem. 274, 12692 (1999). 7. Gamp, AC. et al. Hum. Mol. Genetic. 12, 631 (2003).

Additional Info

Recognizes human LAMP-1. Species Crossreactivity: mouse and rat.