Lecithin Cholesterol Acyltransferase (LCAT)

Lecithin cholesterol acyltransferase (LCAT) is the plasma enzyme responsible for most HDL cholesteryl esters in human plasma. In species with cholesteryl ester transfer protein (CETP) it is also responsible for a significant proportion of VLDL and LDL cholesteryl esters. LCAT, through the esterification of cholesterol, plays a role in the reverse cholesterol transport pathway by facilitating the net movement of cholesterol from peripheral tissues back to the liver for excretion. LCAT deficiency results in low levels of plasma HDL, whereas a transgenic overexpression of LCAT results in increased plasma HDL concentrations.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 detects a band at 62-65kD\nELISA: 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive control: \nLipoprotein fraction of whole human plasma (albumin has the same ~MW as LCAT).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

L1662-50

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Chisholm, J., et al. Characterization of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase. Journal of Lipid Research. 40: 1512-1519, 1999. 2. Furbee, J.W., et al. In vivo contribution of LCAT to apolipoprotein B lipoprotein Cholesteryl esters in LDL receptor and apolipoprotein E knockout mice. Journal of Lipid Research. 43(3): 428-437, 2002. 3. Furbee, J.W., et al. Lecithin:cholesterol acyltransferase deficiency increases arteriosclerosis in the low density lipoprotein receptor and apolipoprotein E knockout mice. J. Biol. Chem. 277(5): 3511-3519, 2002.

Additional Info

Recognizes human Lecithin cholesterol acyltransferase (LCAT). Does not react with mouse. Exhibits neutralizing activity for chloresterol esterification