LIG3 pAb

DNA ligase is a type of ligase that can link together DNA strands that have double-strand breaks. DNA ligase functions in both DNA repair and DNA replication. It is utilized in molecular biology laboratories for recombination experiments. In mammals, the four specific types of DNA ligase are known as DNA Ligase I, II, III and IV. DNA Ligase I ligates Okazaki fragments during lagging strand DNA replication and some recombinant fragments. DNA Ligase II is an alternatively spliced form of DNA Ligase III found in non-dividing cells. DNA ligase III complexes with the DNA repair protein XRCC1 to function in sealing base excision mutations and recombinant fragments. DNA Ligase IV complexes with XRCC4 and catalyzes the final step in the non-homologous end joining DNA double-strand break repair pathway.

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SPECIFICATIONS

Catalog Number

BS6653

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human LIG3.

Species

LIG3 pAb detects endogenous levels of LIG3 protein.

Dilutions

WB: 1:500~1:2000\nIHC: 1:50~1:200

Swiss Prot

P49916

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 103 kDa

Alternative Names

D11Wsu78e; DNA ligase; DNA ligase 3; DNA ligase III; DNLI3; LIG 3; LIG2; LIG3; Ligase II; DNA; ATP dependent; Ligase III DNA ATP dependent; MGC78176; OTTMUSP00000000875; Polydeoxyribonucleotide synthase [ATP] 3; Polydeoxyribonucleotide synthase [ATP]; Polydeoxyribonucleotide synthase; RP23-316C11.6;