LSD1 (Lysine-specific Histone Demethylase 1A, BRAF35-HDAC Complex Protein BHC110, Flavin-containing Amine Oxidase Domain-containing Protein 2, KDM1A, AOF2, KDM1, KIAA0601)

LSD1 a recently identified, first known lysine-specific histone demethylase, is an 886aa nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports suggest that it plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2 it serves as a novel biomarker predictive for prostate cancer.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry (paraffin): 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L4595-50Q

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity Chromatography.

Isotype

IgG

References

1. Stavropoulos, P. et al. Nat. Struct. Mol. Biol. 13:626-632 (2006). 2. Lee, MG. et al. Nature. 437:432-435 (2005). 3. Forneris, F. et al. FEBS. Lett. 579:2203-2207 (2005). 4. Chen, Y. et al. Proc. Natl. Acad. Sci. USA. 103:13956-13961 (2006).

Additional Info

Recognizes human LSD1. Species Crossreactivity: canine, mouse, rat, rhesus monkey.