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LSD1 (Lysine-specific Histone Demethylase 1A, BRAF35-HDAC Complex Protein BHC110, Flavin-containing Amine Oxidase Domain-containing Protein 2, KDM1A, AOF2, KDM1, KIAA0601)

Cat no: L4595-50R

LSD1 (Lysine-specific Histone Demethylase 1A, BRAF35-HDAC Complex Protein BHC110, Flavin-containing Amine Oxidase Domain-containing Protein 2, KDM1A, AOF2, KDM1, KIAA0601)

LSD1 is a flavin-containing novel nuclear protein having an N-terminal SWIRM domain and a C-terminal FAD-binding motif and an amine oxidase domain. It functions as a transcriptional corepressor and catalyzes the flavin-dependent demethylation of Lys4 of histone3 resulting in the formation of methyl-free lysine and release of formaldehyde. LSD1 has been found to be typically associated with CoREST and histone deacetylases 1 and 4 and participates in the silencing of endogenous neuron-specific genes.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBrain lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L4595-50R

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, ZF/Fish

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Shi et.al Cell 119 (7) 941-953 (December 29, 2004) 2. Forneris et.al FEBS Lett 579 (10) 2203-2207 (April 11, 2005).

Additional Info

Recognizes human LSD1. Species Crossreactivity: canine, mouse, rat, rhesus monkey, zebrafish.

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