LSD1 (Lysine-Specific Histone Demethylase, Amine Oxidase Flavin-containing Domain Protein 2, AOF2)

Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear homolog of amine oxidases and functions as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct lysine residues in histone proteins. For example, methylation of histone H3 Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 (Lys9) facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex, which contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 Lys4 via an FADdependent oxidation reaction, and facilitates the repression of neuronal-specific genes in non-neuronal cells (1,6,7). In contrast, when LSD1 is associated with androgen-receptor in human prostate cells, it demethylates mono-methyl and di-methyl histone H3 Lys9 and facilitates androgen-receptordependent transcriptional activation (8). Therefore, LSD1 can function as a co-repressor or co-activator, depending on gene context. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).\n\nApplications: \nSuitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern blot 1:1000\nImmunoprecipitation 1:25\nImmunohistochemistry (Paraffin) 1:200\nIHC Protocol: Citrate/TBST\nImmunofluorescence (IF-IC) 1:100\nFlow Cytometry 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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