LSP1, Phosphorylated (Ser252) (Lymphocyte-specific Protein 1, 47kD Actin-binding Protein, 52kD Phosphoprotein, pp52, Lymphocyte-specific Antigen WP34, WP34)

Leukocyte-specific protein (LSP; also known as Lymphocyte- specific protein, SP34 and pp52) is a 52kD filamentous-actin- binding intracellular phosphoprotein. Human LSP1 is composed of 339aa, which expressed in normal and transformed B- lineage cells and normal T-lymphocytes. Studies show that LSP1 plays an important role in cytoskeletal architecture and motility regulation as well as receptor-induced apoptosis. Research show that LSP1 has at least three and potentially a fourth F-actin binding domain located in the basic C-terminal half. This F-actin binding sites are also correspond to the caldesmon and the villin headpiece proteins. LSP1 246-295 containing sequences homologous to caldesmon F-actin binding site II (CII) can bind F-actin.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L2030-01A

Size

25ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Li Y et al (2000) Blood. 96(3):1100-1105. 2. Howard T. (1998) Blood. 91(12): 4786-4795. 3. Jongstra-Bilen J. et al (2000) Blood. 96(5):1827-1935. 4. Jongstra-Bilen J. et al (1999) Mol. Immunol 36(6):349-359. 5. Klein D. et al (1990) J. Immunol 145(9):2967-2973 6. Jongstra-Bilen J. et al (1992) 118(6):1443-1453. 7. Zhang Q. et al (2000) J. Immunol 2053-2058.

Additional Info

Recognizes human LSP1, when phosphorylated at Serine 252. Species Crossreactivity: mouse.