Luciferase (HRP)

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested. \n\nRecommended Dilution: \nELISA: 1:50,000-1:200,000. Assayed against 1ug of Luciferase [Photinus pyralis (Fire?y)] in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT.\nWestern Blot: 1:5000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

L5999-25

Size

5mg

Applications

ELISA, WB

Hosts

Goat

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with HRP.

P Type

Pab

Purity

Puri?ed from monospeci?c antiserum by delipidation, fractionation and ion exchange chromatography.

Isotype

IgG

Additional Info

Recognizes Luciferase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum and purified and partially purified Luciferase [Photinus pyralis (Fire?y)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.