Ly-6C (Ly6C, Lymphocyte Antigen 6C2, Ly6c2, Ly-6C2, AA682074, AA959465)

The antigen is a 14kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma. In tissue sections, the antigen is found on macrophage precursor subpopulations. In the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen.\nThe monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti-F4/80) is recommended. In combination with an anti-mouse CD31/PECAM-1 antibody, ER-MP20 can be used to evaluate the cellular composition in murine bone marrow (e.g. using flow cytometric analysis). ER-MP20 also detects a wide range of endothelial cells.\n\n\nApplications:\nSuitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry (frozen, paraffin): 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L7715-34G

Size

100ug

Applications

FC, IHC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in 0.02% sodium azide, 0.1% BSA.

P Type

Mab

Purity

Purified

Isotype

IgG2a

References

1. de Bruijn, M et al; Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens. Eur J Immunol 1994, 24: 2279 2.

Additional Info

Recognizes specifically mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes).

Alternative Names

Lymphocyte Antigen 6 Complex, Locus C1, Ly6c1