Lymphoid Enhancer Factor 1, HMG Binding Domain (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)

Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF- 4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.\n\nApplications: \nSuitable for use in Western Blot, Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nPositive Control: CCRF-HSB-2 (human leukemic lymphoblast) cell lysate, HuT 78 (cutaneous T lymphocyte) whole cell lysate, or Jurkat whole cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L8005-10F

Size

250ug

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a sterile-filtered liquid in PBS, pH 7.2, 0.08% sodium azide.

P Type

Mab

Purity

Purified ascites

Isotype

IgG1

References

Eastman Q & Grosschedl R Regulation of LEF-1/TCF transcription factors by Wnt and other signals. Curr Opin Cell Biol 11:233-40 (1999). <PUBMED:10209158>

Additional Info

Recognizes the High Mobility Group DNA domain of human LEF-1. Species Crossreactivity: mouse.