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Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)

Cat no: L8005-10J

Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)

The human T cell-specific transcription factor LEF-1/TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions (1). TCF-1 alpha participates in the regulation of TCR alpha enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. The function of LEF-1/TCF-1 is dependent, in part, on the HMG domain that induces a sharp bend in the DNA helix, and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins (2). Constitutive activation of the Wnt signaling pathway is a root cause of many colon cancers. Activation of this pathway is caused by genetic mutations that stabilize the beta-catenin protein, allowing it to accumulate in the nucleus and form complexes with any member of the lymphoid enhancer factor and T-cell factor family of transcription factors (referred to collectively as LEF/TCFs) to activate transcription of target genes (3).\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000-20,000\nImmunohistochemistry: 1:100-1:250\nImmunocytochemistry: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

L8005-10J

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernantant

Isotype

IgG

References

1.Waterman ML, et al. Genes Dev 5(4):656-69, 1991 2.Bruhn L, et al. Genes Dev 11(5):640-53, 19973.Hovanes K, et al. Nat Genet 28(1):53-7, 2001

Additional Info

Recognizes human LEF-1.

Alternative Names

LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha

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