Lysine, Acetylated (Acetyl-Lysine)

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipation, Chromatin Immunoprecipitation, Immunohistcohemistry, Immunofluorescence and ELISA. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000 \nImmunoprecipitation: 1:100 \nChromatin IP: 1:50\nImmunohistochemistry (paraffin): 1:1600\nImmunofluorescence (IF-IC): 1:200\nELISA (peptide): 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L9058-01

Size

100ul

Applications

ELISA, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1. Hassig, C.A. and Schreiber, S.L., Curr. Opin. Chem. Biol. 1(3): 300-308 (1997). 2. Allfrey, V.G., et al., Proc. Natl. Acad. Sci. USA 51: 786-794 (1964). 3. Liu, L., et al., Mol. Cell. Biol. 19(2): 1202-1209 (1999). 4. Boyes, J., et al., Nature 396: 594-598 (1998). 5. Polevoda, B. & Sherman, F., Genome Biol. 3: reviews00006 (2002). 6. Yoshida, M., et al., Prog. Cell. Cycle Res. 5: 269-278 (2003). 7. Kim, S.C., et al., Mol. Cell 23: 607-618 (2006). 8. Choudhary, C., et al., Science 325: 834-840 (2009). 9. Hughes, R.E., Curr. Biol. 12: R141-143 (2002). 10. Vigushin, D.M. & Coombes, R.C., Curr. Cancer Drug Targets 4: 205-218 (2004).

Additional Info

Recognizes proteins post-translationally modified by acetylation on the epsilon-amino groups of lysine residues. Recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF and chemically acetylated BSA. Has been shown to react with as little as 0.04ng of chemically acetylated BSA while not recognizing up to 25ug of nonacetylated BSA.