Lysophospholipase D (LysoPLD)

Lysophosphatidic acid (LPA) is an extracellular signaling lipid that evokes multiple biological functions including induction of platelet aggregation, smooth muscle contraction, and stimulation of cell proliferation and chemotaxis. Lysophospholipase D (LysoPLD) was first discovered in 1999 as the enzyme responsible for generating LPA from lysophosphatidylcholine (LPC). It was later revealed to be identical to an autocrine motility factor, autotaxin (ATX), which plays a role in tumor progression and metastasis. LysoPLD/ATX mRNA is widely expressed with highest levels found in brain, ovary, lung, intestine, and testis. Rat LysoPLD is composed of 885 amino acids with an estimated molecular weight of 101kD. The protein is reported to be heavily glycosylated and thus its apparent size on SDS-PAGE may be run as high as 125kD. Useful positive controls include cerebrospinal fluid, mouse ascites or seminal plasma.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 5ug/ml\nImmunofluorescence (IF-IC): 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L9189-65

Size

100ul

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from TBS, pH 7.4. Reconstitute with 500ul sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ishii, I., et al., Annu. Rev. Biochem. 73: 321-354 (2004). 2. Tokumura, A., et al., J. Biol. Chem. 277(42): 39,436-39,442 (2002). 3. Umezu-Goto, M., et al., J. Cell Biol. 158: 227-233 (2005). 4. Nam, S.W., et al., Oncogene 19: 241-247 (2000). 5. Sun, Y., et al., J. Biochem. 380: 749-755 (2004). 6. van Leeuwen, F.N., et al., Biochem. Soc. 31: 1209-1212 (2003). 7. Murata, J., et al., J. Biol. Chem. 269(48): 30,479-30,484 (1994).

Additional Info

Recognizes rat LysoPLD. Species Crossreactivity: human and mouse.