Lysosome-associated Membrane Protein 1 (LAMP1, LAMP-1, Lysosome-associated Membrane Glycoprotein 1, CD107 Antigen-like Family Member A, CD107a)

LAMP1 is a protein primarily associated with the lysosomal membrane. Antibodies to LAMP1 are markers of lysosomes in mammalian cells, though some LAMP1 may also be seen on late endosomes and on the plasma membrane. In a typical cell LAMP1 is associated with spherical vesicles located next to the nucleus and the microtubule organizing center (1). The protein is also known as CD107a, LGP120 and LAMPA. LAMP1 is found on the cell surface of lymphocytes undergoing degranulation and this phenomena resulted in discovery of LAMP1 as a CD protein. The LAMP1 protein has a large N-terminal region which is inside the lysosome, hence topologically external to the cell, which is often refered to as the lumenal domain (2). The lumenal domain consists of two homologous globular seqments separated by a proline rich sequence. There is a single membrane spanning domain and a short 11 amino acid C-terminal cytoplasmic tail. This tail region contains a so-called YXXI motif which is responsible for the sorting of the intact molecule to the endosome and lysozome, where Y = tyrosine, I = isoleucine and X = almost any amino acid (3). This motif is found in several other lysosomal proteins, where it functions in the same way. There are 417 amino acids in the human LAMP1 molecule, giving a native molecular weight of 44.8kD. The N-terminal lumenal segment of LAMP1 is very heavily and variably glycosylated due to the presence of 18 N-linked glycosylation sites, so that on SDS-PAGE and on Western Blots the protein runs as a diffuse band at 90-120kD.\n\nApplications:\nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:2000\nWestern Blot: 1:10,000 using chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031384

Size

100ul

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Prc

Form

Supplied as a liquid in PBS, 10mM sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1

References

1. Matteoni, R. et al.(1987) J. Cell Biol. 105:1253-1265. 2. Howe CL, et al. (1988) Proc Natl Acad Sci USA. 85:7577-81. 3. Rohrer J, et al. (1996) J Cell Biol. 132:565-76 .

Additional Info

Recognizes LAMP1 from human, bovine, porcine, mouse, rat and other mammals. Since LAMP1 is highly conserved, it is likely that the antibody is effective on other species also.