Lysosome Associated Membrane Protein 2 (LAMP 2, CD107b, Igp2, Igp110)

Lysosomes are membrane-bound organelles found within the cytoplasm of most cells, which contain hydrolytic enzymes and act as the major compartment for heterophagic and autophagic digestion. Members of the lysosomal-associated membrane protein family (LAMPS) are believed to play an important role in protecting the lysosomal membrane from protease degradation and are involved in lectin-mediated cell adhesion. Evidence shows that CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway and human studies have revealed a correlation between the level of surface expression of CD107b on tumor cells and their metastatic potential.\n\nApplications: \nSuitable for use in Western Blot, Electron Microscopy, Immunoprecipitation, Immunofluorescence and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:100-1:500\nFlow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required for this application. \nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nSpleen cells from immunized BALB/c mice were fused with cells of the NS1 myeloma cell line. \n\nRecommended Negative Control:\nI1904-78R: IgG1 Murine Negative Control\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L9190-15D

Size

250ug

Applications

FC, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Nabi, I.R., et al., J. Cell Biol. 115: 1573-1584 (1991). 2. Nabi, I.R. and Rodriguez-Boulan, E., Mol. Biol. Cell 4: 627-635 (1993). 3. Tzuu-Shuh, J., et al., Mol. Biol. Cell 11: 287-304 (2000). 4. Ihrke, G., et al., EMBO. J. 22: 6256-6264 (2001). 5. Fukuda, M., J. Biol. Chem. 266: 21,327-21,330 (1991). 6. Pluhar, G.E. et al (2010) Anti-tumor immune response correlates with neurological symptoms in a dog with spontaneous astrocytoma treated by gene and vaccine therapy. Vaccine. 28: 3371-8. 7. Cliffe, S.T. et al. (2009) SLC29A3 gene is mutated in pigmented hypertrichosis with insulin-dependent diabetes mellitus syndrome and interacts with the insulin signaling pathway. Hum Mol Genet. 18: 2257-65.

Additional Info

Recognizes canine CD107b, a 95kD heavily-glycosylated type I integral lysosomal glycoprotein. Demonstrates staining pattern consistant with localization to lysosome.\nSpecies Crossreactivity: human and mink. Does not react with mouse or rat.