Lysosome Associated Membrane Protein 2 (LAMP 2, CD107b, Igp2, Igp110) (PE/CY5)

CD107b, also known as lysosomal-associated membrane protein 2 (LAMP-2), is a heavily glycosylated, type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107b is expressed by granulocytes, T cells, macrophages, dendritic cells, activated platelets tonsillar epithelium and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-2 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential, where they may increase adhesion to extracellular matrix and endothelium.1-7\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry (acetone-fixed, frozen tissue sections). Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul/10e6 cells\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L9190-14E

Size

100Tests

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with PE/CY5.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD107a, 107b Section, Academic Press, New York, p. 389. 3. Sawada, R., K.A. Jardine, and M. Fukuda. 1993. J. Biol. Chem. 268:9014. 4. Febbraio, M., and R.L. Silverstein. 1990. J. Biol. Chem. 265:18531. 5. Chen, J.W., Y. Cha, K.U. Yuksel, R.W. Gracy, and J.T. August. 1988. J. Biol. Chem. 263:8754. 6. Sawada, R., J.B. Lowe, and M. Fukuda. 1993. J. Biol. Chem. 268:12675. 7. Sawada, R., S. Tsuboi, and M. Fukuda. 1994. J. Biol. Chem. 269:1425.

Additional Info

Recognizes human LAMP 2.