Lysosome Associated Membrane Protein 2 (LAMP 2, Igp2, Igp110)

Lysosome-associated membrane protein 2 (LAMP 2), also known as lgp2 or lgp110, is a type 1 integral membrane protein that is transported from the trans-Golgi network to endosomes and then lysosomes (1). LAMPs, also known as lysosomal integral membrane proteins (LIMPs), are ubiquitous, highly glycosylated and account for ~50% of lysosomal membrane protein; their function is largely unknown. All LAMPs have a short, 10-20 amino acid, cytosolic domain which contain single tyrosine or di-leucine motifs, that interact with adaptor complexes (APS) for sorting at the trans-Golgi network and targeting to lysosomes (2). LAMP 2 has also been detected at the plasma membrane of cells in a differentiation and activation dependent manner, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expression patterns of membrane LAMP 2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (3). Cell surface LAMP 1 and 2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, possibly involved in the adhesion of PBMC to the site of inflammation (4). Studies with LAMP 2 deficient mice indicate LAMP 2 is critical for autophagy, in conversion of early autophagic vacuoles to vacuoles, which rapidly degrade their content (5). It is also the primary defect in Danon disease (2,5).\n \nApplications:\nSuitable for use in Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nImmunocytochemistry: 1:50. Labels the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponn. \nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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